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疼痛与炎症研究

甩尾仪

来源:本站作者:登录九游会仪器

详细介绍

热甩尾仪根据D"amour/Smith原理和方法,测量大鼠、小鼠尾巴部受红外热刺激时的痛觉阈值。


实验步骤及方法:

· 该仪器使用可调强度的红外光源,红外光通过一抛物镜面反射聚焦在实验动物的尾巴上;

· 操作人员将实验动物置于仪器上,把动物的尾巴放在红外光源处,接受热辐射刺激;

· 当动物感觉到疼痛时,尾巴会左右甩动或者轻敲台面,内置传感器会立刻检测到尾巴的活动,停止计时和关闭光源;

· 仪器自动记录反应时间和光源强;

· 数据可通过U盘或USB数据线导出到电脑;

· 该型号的设备在世界上使用和验证超过3000次,积累了数十年的经验;



型号:37560


主要特点:

· 尾部轻拍可自动记录或手动评分;
· 自动化程度高,避免人为因素引起的误差;
· 触摸屏控制,操控方便,显示直观;
· 可独立工作,也可连接电脑使用;
· 测试平台平整,表面无突出和遮挡部件;
· 可选配红外热辐射校准仪用于校准红外光源;
· 多种单位可转换;

· 该仪器适合大鼠和小鼠的测试;

· 有大鼠、小鼠两种不同的固定器可供选择;


正在进行大鼠甩尾测试


仪器的操作
· 主机内包括红外光源、传感器、微控制器;
· 自动检测尾巴的活动情况,自动计时,延迟时间会被自动记录;
· 可选配倾斜的老鼠固定器,将老鼠尾巴保持在 45 度向上的位置




可选配的小鼠固定器



控制面板


数据采集
· 数据会存储设备中,可以通过USB接口直接导出到电脑;
· 基于CUB Windows®的软件,可实现数据的传输;

· 数据格式通用化,可使用其他软件进行统计;
· 带储存功能,用于实验数据的保存;

· 可联网,通过远程网络对实验方案进行编辑;

校准辐射计
· 用来对辐射强度进行校准;校准辐射计货号我37300;
· 确保两个或多个装置提供完全相同强度的热伤害性刺激(辐射单位:mW/cm2)
· 测量红外光的能量的绝对值(1s持续时间内的1mW对应1mJ


   主要参数
 电源  通用 85-264 VAC, 50-60Hz
 控制   软键和脚踏板
 数据读取  多功能的图像显示
 打印  微型热敏打印机(需另外购买)
 红外强度  01-99可调 
 反应时间  液晶屏显示,分辨率为0.1s
 数据传输  USB接口
 校准  红外热辐射计(需另外购买)
 工作温度  10-40°C
 重量  4Kg
 尺寸  43x22x10cm 
   标准配置
 37360  甩尾仪主机
 37215-303  脚踏板
 E-AU 041  存储卡,包含以下
 37370-302  安装说明书
 52050-10  数据采集软件包
 52010-323  USB 数据线
 E-HR 002

 替换灯

 (Halogen"Bellaphot",Mod.64607 OSRAM,8V-50W)

 E-US 063-1  电池 M4T32-BRI2SH1
   额外选配
 37360-325  小鼠束缚器 (25mm I.D.)
 37360-330  小鼠束缚器(30mm I.D.)
 37300  红外热辐射计
 57145  微型打印机


参考文献:

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12.Suzuki T, Sawada T, Kawai K, Ishihara Y. Pharmacological profile of TAN-452, a novel peripherally acting opioid receptor antagonist for the treatment of opioid-inducedbowel syndromes. Life Sci. 2018;215:246-252. doi:10.1016/j.lfs.2018.07.028(IF=6.1)

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14.Palazzo E, Boccella S, Marabese I, et al. Homo-AMPA in the periaqueductal grey modulates pain and rostral ventromedial medulla activity in diabetic neuropathic mice. Neuropharmacology. 2022;212:109047. doi:10.1016/j.neuropharm.2022.109047(IF=4.7)

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16.Sasaguri T, Taguchi T, Murata Y, et al. Interleukin-27 controls basal pain threshold in physiological and pathological conditions. Sci Rep. 2018;8(1):11022. Published 2018 Jul 23. doi:10.1038/s41598-018-29398-3(IF=4.6)

17.Komatsu A, Miyano K, Nakayama D, et al. Novel Opioid Analgesics for the Development of Transdermal Opioid Patches That Possess Morphine-Like Pharmacological Profiles Rather Than Fentanyl: Possible Opioid Switching Alternatives Among Patch Formula. Anesth Analg. 2022;134(5):1082-1093. doi:10.1213/ANE.0000000000005954(IF=5.7)

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方法学文献:

F.E. D’mour & D.L. Smith: "." J. Pharmacol. Exp. Therap. 72: 74-79, 1941.




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